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(一)用差示离心方法,由大白鼠肝得到的颗粒体制剂在谷胱甘肽的保护作用下,有将 L-古罗糖酸-或 L-半乳糖酸-γ-内酯氧化为 L-抗坏血酸的作用,活力大部存在于微粒体。(二)利用脱羟胆盐处理微粒体可使之成为局部溶解的酶制剂,经氧化铝凝胶 C_r 吸附,硫酸铵沉淀续加纯化,活力提高5—6倍。(三)对于酶作用的一般情况和一些因素对它的影响,我们作了一些观察。(四)比较并讨论了我俩所得结果与其他工作者所报告者的异同。
(A) by differential centrifugation, particle preparations obtained from the liver of rats in the protective effect of glutathione, L-gulonic acid or L-galactono-γ-lactone oxidation to The role of L-ascorbic acid, vitality most of the microsomes. (B) the use of hydroxy acid treatment of microsomal bile salts can be made into a local enzyme solution dissolved in alumina gel C_r adsorption, ammonium sulfate precipitation continue to add purification, increased vitality 5-6 times. (C) For the general situation of the enzyme and the impact of some factors on it, we made some observations. (Iv) Compare and discuss the similarities and differences between the results we obtained and those reported by other workers.