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作者应用由冈比亚按蚊的核糖体DNA(rDNA)顺反子克隆化的0.59Kb DNA片段作探针,鉴别冈比亚按蚊复合体中形态相同的3个亲缘种冈比亚按蚊、阿拉伯按蚊和美拉按蚊(An.melas)。从冈比亚按蚊G-3株的成蚊提取DNA,在琼脂糖凝胶板上电泳洗脱提取13～20Kb
The authors used a 0.59 kb DNA fragment cloned from the ribosomal DNA (rDNA) cistron of Anopheles gambiae as a probe to identify three morphologically related Anopheles gambiae Anopheles gambiae, An.melas. DNA was extracted from adult mosquitoes of Anopheles gambiae G-3 strain and electrophoresed on an agarose gel plate to extract 13-20 kb