1989年8月起进行了中国对虾（Penaeuschinensis）上皮组织的原代和传代细胞培养。对取自甲壳、步足、尾扇等部位的对虾上皮组织进行了组织培养研究，共培养6批，获得传代细胞11株，其中1株传至25代，传代细胞形态为梭形、椭圆、多边形，和正常上皮细胞形态相似。生长率测定结果，第4天细胞增殖达高峰，倍增时间为48．72h。 From August 1989, primary and subculture of Penaeus chinensis epithelial cells was performed. Tissue culture of shrimp epithelial tissue extracted from carapace, step foot, tail fan and other parts of the shrimp were studied. Six batches were co-cultured and 11 passaged cells were obtained, of which 1 was passaged to 25 passages. The passaged cells were spindle, ellipse, Polygons, similar to normal epithelial cells. Growth rate determination results, the first 4 days of cell proliferation reached a peak, doubling time was 48.72h.