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目的:观察牛膝总皂苷对过氧化氢(H2O2)诱导人脐静脉内皮细胞(HUVEC)损伤的保护作用。方法:体外培养HUVEC,分为空白对照组、模型对照组及牛膝总皂苷低、中、高浓度组,MTT法检测细胞活力,微板法检测上清液中一氧化氮(NO)的含量,采用ELISA法检测内皮素-1(ET-1)、组织型纤溶酶原激活物(tPA)、纤溶酶原激活物抑制剂(PAI-1)的含量。结果:与模型对照组比较,牛膝总皂苷中、高浓度组细胞活力显著升高,ET-1和PAI-1含量显著减少,NO和tPA含量显著升高(P<0.05或P<0.01)。结论:牛膝总皂苷对H2O2诱导的HUVEC损伤具有显著的保护作用。
Objective: To observe the protective effect of total saponins of Achyranthes bidentata (HYD) on the injury of human umbilical vein endothelial cells (HUVEC) induced by hydrogen peroxide (H2O2). Methods: HUVECs were cultured in vitro. The cells were divided into blank control group, model control group and low, medium and high concentration of Achyranthes bidentata total saponins. Cell viability was detected by MTT assay. Nitric oxide (NO) The levels of endothelin-1 (ET-1), tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor (PAI-1) were determined by ELISA. Results: Compared with the model control group, the cell viability of ET-1 and PAI-1 were significantly increased and the content of NO and tPA were significantly increased (P <0.05 or P <0.01) . Conclusion: Achyranthes saponin has a significant protective effect on HUVEC induced by H2O2.