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In angiosperms, pollen development is an intricate indispensable process for successful sexual reproduction, which involves accurately and tightly controlled regulations and requires numerous genes expressed in both sporophytic and gametophytic tissues of the anther (McCormick, 2004).Understanding the molecular mechanism underlying pollen development is of great significance for the construction of male sterile lines and crop breeding.However, many critical issues concerning pollen development still need to be elucidated.Recently, targeted editing of pollen development-related genes mediated by CRISPR/Cas9 system has provided powerful tools to facilitate the relevant studies in crops to figure out these issues (Li et al., 2016, 2017).