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本研究旨在建立可用于活体成像的小鼠肺癌移植瘤模型。利用脂质体将荧光素酶表达载体pGL4.17(luc2/neo)转染至人非小细胞肺癌细胞株A549,经G418筛选获得稳定表达荧光素酶的细胞克隆。根据体外生物发光情况及细胞的生长特性,从中挑选合适克隆,进行裸鼠皮下接种,SCID鼠尾静脉接种,建立肺癌移植瘤模型。利用活体成像系统监测肿瘤的生长转移情况,并用切片HE染色进一步验证小鼠模型移植瘤的原位成瘤和转移能力。实验结果表明:本研究成功地构建了可用于活体成像的小鼠肺癌移植瘤模型,模型稳定可靠、直观、灵敏,为肿瘤生长转移机制的研究及抗肿瘤药物的研发提供了重要工具。
This study aimed to establish a mouse lung cancer xenograft model that could be used for in vivo imaging. The luciferase expression vector pGL4.17 (luc2 / neo) was transfected into human non-small cell lung cancer cell line A549 by lipofectamine and the cell clone stably expressing luciferase was obtained by G418 screening. According to the bioluminescence in vitro and the growth characteristics of the cells, the suitable clones were picked out for subcutaneous inoculation of nude mice and tail vein inoculation of SCID mice to establish a lung cancer xenograft model. The growth and metastasis of the tumor was monitored by the live imaging system, and the in situ tumorigenesis and metastasis ability of the mouse model xenografted tumor was further verified by section HE staining. The experimental results show that this study successfully constructed a mouse lung cancer xenograft model that can be used for in vivo imaging. The model is reliable, intuitive and sensitive. It provides an important tool for the study of tumor growth and metastasis mechanisms and the development of anticancer drugs.