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采用田间试验,研究了不同氮肥水平(0,30,60,90 kg·hm-2)下大豆VE、R1、R3、R5、R7期根际土壤细菌、放线菌及真菌含量的变化及对大豆产量的影响。结果表明:大豆根际土壤中细菌、真菌和放线菌数量随着施氮量的增加而增加;细菌和真菌数量随生育时期的推进呈先上升后略有下降的趋势,并在R5期达到最大值;大豆产量随施氮量的增加先升高再降低,其中60 kg·hm-2处理效果最好,产量为2 734.2 kg·hm-2,较不施肥增产13.0%。因此,施入适量氮肥有利于提高土壤微生物总量和大豆产量。
Field experiments were conducted to study the changes of soil bacteria, actinomycetes and fungi in the rhizosphere soils of VE, R1, R3, R5 and R7 at different nitrogen levels (0, 30, 60 and 90 kg · hm-2) Effect of soybean production. The results showed that the number of bacteria, fungi and actinomycetes in rhizosphere soil increased with the increase of nitrogen application rate. The number of bacteria and fungi increased slightly and then decreased slightly with the advancing stage and reached the level of R5 Maximum; Soybean yield first increased and then decreased with the increase of N application rate, of which 60 kg · hm-2 treatment effect was the best, the yield was 2 734.2 kg · hm-2, 13.0% higher than no fertilization. Therefore, the application of nitrogen fertilizer is conducive to increase the total amount of soil microorganisms and soybean production.