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[目的]探讨凋亡素基因(VP3)在卵巢癌细胞株CoC1及CoC1细胞耐药亚株CoC1/DDP中的诱导凋亡情况。[方法]构建重组凋亡素真核表达载体pcDNA3.1-VP3。在体外将pcDNA3.1-VP3转染入卵巢癌细胞株CoC1及CoC1细胞耐药亚株CoC1/DDP中,RT-PCR检测凋亡素在细胞中的表达,用流式细胞仪检测细胞凋亡。[结果]RT-PCR证实VP3基因在CoC1及CoC1/DDP中存在并在转录水平表达。转染pcDNA3.1-VP3细胞凋亡率明显高于转染空质粒组(P<0.01),转染pcDNA3.1-VP3细胞凋亡率CoC1/DDP细胞明显高于CoC1细胞(P<0.01)。[结论]凋亡素诱导人卵巢癌细胞CoC1和CoC1顺铂耐药亚株CoC1/DDP凋亡,且更易诱导CoC1顺铂耐药亚株CoC1/DDP细胞的凋亡。
[Objective] To investigate the apoptosis induction of apoptosis gene VP3 in CoC1 and CoC1 resistant CoC1 / DDP ovarian cancer cell lines. [Method] The recombinant eukaryotic expression vector pcDNA3.1-VP3 was constructed. In vitro, pcDNA3.1-VP3 was transfected into CoC1 and CoC1 resistant CoC1 / DDP cells. The expression of apoptin in the cells was detected by RT-PCR and the apoptosis was detected by flow cytometry . [Result] RT-PCR confirmed that VP3 gene existed in CoC1 and CoC1 / DDP and expressed at the transcriptional level. The apoptosis rate of transfected pcDNA3.1-VP3 cells was significantly higher than that of the transfected empty plasmid group (P <0.01). The apoptosis rate of CoC1 / DDP cells transfected with pcDNA3.1-VP3 cells was significantly higher than that of CoC1 cells (P <0.01) . [Conclusion] Apoptosis induces the apoptosis of CoC1 / DDP cells and inhibits the apoptosis of CoC1 / DDP cells.