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目的 检测饮食服务人群HBV感染状况 ,研究HBVDNA阳性者的家庭聚集性 ,探讨其传染性 ,为修订相应法规提供依据。方法 用反向间接血凝法检测HBsAg ,用赖氏法检测ALT ,酶联免疫吸附试验检测HBVM ,聚合酶链反应 微孔杂交法和荧光定量PCR法检测HBVDNA。对HBVDNA阳性者的家庭聚集性进行对照研究。结果 在 14 4 92名饮食服务人员中检出HBsAg阳性 4 96人 ,阳性率 3.4 2 %。其中HBsAg≥ 1∶5 12或ALT >4 0者 137人 ,占阳性者的 2 7.6 2 %。ALT <4 0 ,HBsAg <1∶5 12 ,且HBeAg阳性者 5 5人 ,占 15 .32 %。在HBsAg <1∶5 12、ALT <4 0、且HBeAg阴性的 30 4人中检出HBVDNA阳性者 113人 ,阳性率 34.6 7%。HBVDNA阳性者的家庭聚集率为 86 .4 9% ,家庭成员HBV感染率为 6 4 .38% ,相对危险性 6 .5 8。HBVDNA阳性者的家庭成员HBV感染呈家庭聚集性。结论 血清学检验结果同时表现为HBsAg <1∶5 12、ALT <4 0、HBeAg阴性、符合现行法规要求 ,但HBVDNA阳性的在岗饮食服务人员是HBV的危险传染源。
Objective To detect the status of HBV infection in diet service population, to study the family aggregation of HBVDNA positive individuals and to explore their contagion, so as to provide the basis for the revision of relevant laws and regulations. Methods HBsAg was detected by reverse indirect hemagglutination, ALT by Lai’s method, HBVM by enzyme-linked immunosorbent assay (ELISA), micro-hybridization by polymerase chain reaction (PCR) and fluorescence quantitative PCR. A controlled study of familial aggregation in HBVDNA positive individuals. Results A total of 4 96 HBsAg positive cases were detected among 14 492 food service workers, with a positive rate of 3.4 2%. Among them, 137 were HBsAg ≥ 1: 12 or ALT> 40, accounting for 2 7.62% of the positive ones. ALT <40, HBsAg <1: 5 12, and HBeAg-positive 5 5, accounting for 15.32%. Among HBeAg <1: 5 12, ALT <40, HBeAg positive was detected in 30 4 HBeAg negative individuals, with a positive rate of 34.6 7%. The family aggregation rate of HBVDNA positive persons was 86.49%, and the HBV infection rate of family members was 6.44% and the relative risk was 6.58. HBV infection in family members with HBVDNA positive was family clustering. Conclusions The results of serological test showed that both HBsAg <1: 5 12, ALT <40, negative for HBeAg, in line with current laws and regulations, but HBVDNA positive on-the-job food service staff is a dangerous source of HBV infection.