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硝硫氰胺(Amoscante,Amo)是一个强效抗血吸虫药。本文介绍该药在血浆中检测的方法学。本文应用高效液相色谱(HPLC)254nm单波长紫外检测定量分析方法和薄层色谱(TLC)方法分离检测血浆中的Amo。HPLC的条件是:ODS柱,洗脱液为甲醇:水:氯仿=70:20:10,内标物为1,2,3,4-四氢喹啉(THQ)。TLC的条件是:硅胶板,洗脱液为丁醇:石油醚(60:80)=10:90,显色方法为紫外检测和化学检测。建立该药分子中某些基团的特殊化学检测方法可用于鉴定TLC板上的药物,其结果与HPLC分析一致。HPLC和
Nitrosamine (Amoscante, Amo) is a potent anti-schistosome drug. This article describes the methodology for the drug’s detection in plasma. In this paper, high performance liquid chromatography (HPLC) 254nm single-wavelength UV detection quantitative analysis and thin-layer chromatography (TLC) separation and detection of Amo in plasma. HPLC conditions: ODS column, the eluent was methanol: water: chloroform = 70: 20: 10, the internal standard 1,2,3,4-tetrahydroquinoline (THQ). TLC conditions are: silica gel plate, the eluent is butanol: petroleum ether (60:80) = 10: 90, the color method for UV detection and chemical detection. A special chemical assay to establish certain groups in the drug molecule can be used to identify the drug on the TLC plate, the result of which is consistent with HPLC analysis. HPLC and