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目的筛选长萼鸡眼草的高抗氧化活性萃取部,测定其总酚和总黄酮的含量。方法长萼鸡眼草乙醇提取物分别用石油醚、氯仿、乙酸乙酯、正丁醇萃取,利用清除OH.、O2-.和DPPH.实验以及还原能力等方法评价其不同极性萃取部的抗氧化能力。采用Folin-Ciocalteu法测其总酚含量,NaNO2-Al(NO3)3-NaOH显色法测总黄酮含量。结果长萼鸡眼草正丁醇萃取部和乙酸乙酯萃取部的抗氧化活性最强,对OH.和O2-.的清除效果显著高于VitC(P<0.05),石油醚萃取部和氯仿萃取部的抗氧化作用较弱。长萼鸡眼草正丁醇萃取部和乙酸乙酯萃取部的总酚含量分别为105.81±0.25、144.63±0.54 mg.g-1,总黄酮含量分别为199.15±2.63、396.24±5.13 mg.g-1。结论长萼鸡眼草的高抗氧化萃取部为正丁醇萃取部和乙酸乙酯萃取部,其抗氧化活性组分可能为酚酸类和黄酮类化合物,可作为天然抗氧化物质的来源。
OBJECTIVE To screen the extract of high antioxidant activity of Caltha palustris to determine its content of total phenols and total flavonoids. Methods The ethanol extracts of C.cerevisiae were extracted with petroleum ether, chloroform, ethyl acetate and n-butanol, and their anti-extraction activities were evaluated by scavenging OH, O2-, DPPH and reducing power Oxidation ability. Folin-Ciocalteu method was used to determine the total phenolic content, and NaNO2-Al (NO3) 3-NaOH colorimetric method to determine the total flavonoids. Results The antioxidant activity of n-butanol and ethyl acetate extracts of Acremonium chinense was the strongest. The removal efficiencies of OH and O2 were significantly higher than that of VitC (P <0.05) Department of the antioxidant effect is weak. The total phenolic contents of n-butanol and ethyl acetate extracts of C. acutissima were 105.81 ± 0.25 and 144.63 ± 0.54 mg.g-1, respectively, and the total flavonoids contents were 199.15 ± 2.63 and 396.24 ± 5.13 mg.g- 1. CONCLUSION The antioxidant constituents of Calyx oblate are n-butanol extract and ethyl acetate extract. The antioxidative components may be phenolic acids and flavonoids, which may serve as a source of natural antioxidants.