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经典柱层析具有简便易行和上样量较大等优点,故仍为植化常规分离手段。若采用细颗粒载体如青岛硅胶H(10～40μm)装柱,其分离效能显著提高,但溶剂在常压下难以展开洗脱,加压即可行正常柱层析。国内已报道若干
Classical column chromatography has the advantages of simplicity, large sample size, and so on, so it is still a conventional separation method for phytochemicals. If a fine particle carrier such as Qingdao Silica Gel H (10-40 μm) is used for column packing, the separation performance is significantly improved, but it is difficult for the solvent to be eluted under normal pressure, and normal column chromatography can be performed under pressure. There have been several reports in China