论文部分内容阅读
目的:探究黑木耳多糖提取物对肝纤维化大鼠血清髓过氧化物酶(MPO)、丙二醛(MDA)及转化生长因子β(TGF-β)的影响及肝功能的保护作用。方法:将健康的Wistar大鼠30只随机分为3组:对照组、模型组、黑木耳多糖提取物组,利用胆总管结扎术制备肝纤维化动物模型,术后分别用生理盐水0.1 m L/(kg·d)、黑木耳多糖提取物0.1 mg/(kg·d)灌胃1次/d。21 d后腹主动脉采血,测肝功能指标,取肝组织测MDA、MPO、TGF-β,Masson染色。结果:肝纤维化动物模型成功制备。黑木耳多糖提取物组总胆红素(STB)、谷氨酸转移酶(ALT)、天门冬氨酸转移酶(AST)、MPO、MDA较模型组明显降低,TGF-β含量降低。Masson染色可见:模型组肝小叶结构破坏,较对照组汇管区可见大面积胶原纤维的形成;黑木耳多糖提取物组肝细胞轻度水肿、肝索排列欠整齐,肝汇管区及各部位胶原纤维形成量略少。结论:黑木耳多糖提取物可减轻胆总管结扎大鼠脂质过氧化损伤,MPO含量使TGF-β因子释放减少,从而减少胶原纤维形成,减轻或延缓大鼠肝纤维化。
Objective: To investigate the effect of black fungus polysaccharide extract on serum myeloperoxidase (MPO), malondialdehyde (MDA) and transforming growth factor-β (TGF-β) and the protective effect of liver function in rats with liver fibrosis. Methods: Thirty healthy Wistar rats were randomly divided into three groups: control group, model group, black fungus polysaccharide extract group, and hepatic fibrosis animal model by common bile duct ligation. After operation, / (kg · d), black fungus polysaccharide extract 0.1 mg / (kg · d) gavage once / d. Abdominal aorta blood samples were taken after 21 days, and liver function indexes were measured. MDA, MPO, TGF-β and Masson staining were taken from liver tissues. Results: The liver fibrosis animal model was successfully prepared. The content of total bilirubin (STB), glutamic acid transferase (ALT), aspartate aminotransferase (AST), MPO and MDA of the black fungus polysaccharide extract group was significantly lower than that of the model group and the content of TGF-β was decreased. Masson staining showed that the structure of hepatic lobules in the model group was destroyed, and the formation of large-area collagen fibers was observed in the portal area of the model group. The hepatocytes were slightly edematous, Slightly less formation. Conclusion: The polysaccharide extract of Auricularia auricula can relieve lipid peroxidation injury induced by common bile duct in rats. MPO content can reduce the release of TGF-β, reduce the formation of collagen fibers and reduce or delay the hepatic fibrosis in rats.