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种间杂交的障碍在于种间的不亲和性反应,细胞原生质体融合杂交则有可能克服这些不亲和性障碍。CIAT生物工程部使用生物工程技术使分离原生质体在继代培养下,圭亚那笔花豆和头状笔花豆的叶肉细胞的原生质体实现了融合。 CIAT在对两个圭亚那笔花豆品种的细胞培养所作的细胞学的分析表明了染色体在数量上有很大的变异,四倍体、多倍体和非整倍体均已发现出现较高的频率,在目前的这个试验中已明显地表现由愈合组织培养的圭亚那笔花豆的新植株所表现的变异均是通过种子遗传的,对这种变异所带来的对育种的好处尚有待评价。突变体中的一些性状在育种的苗期可作为标记。
The barrier to interspecific hybridization lies in intersexual incompatibility reactions, and cell protoplast fusion crosses make it possible to overcome these incompatibility disorders. The CIAT Bioengineering Division uses bioengineered technology to fuse protoplasts of mesophyll cells isolated from the protoplasts of the detached protoplasts in subcultures of Guyana pena and capitatum. Cytological analyzes by CIAT of cell cultures of two Guyana penicillin cultivars show a large number of chromosome aberrations and tetraploids, polyploids and aneuploids have been found to have high In the present trial, it has been evident that the variability shown by the new plantlets of the Guyana pen-bean cultivated in a healing tissue is inherited by seeds and that the benefits to breeding of this mutation have yet to be evaluated . Some traits in the mutant can be used as markers in breeding seedling stage.