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[目的]通过比较维吾尔族宫颈癌与宫颈非病变组织中Bcl-2、Bcl-xl、c-IAP1表达的差异,了解维吾尔族宫颈癌与凋亡基因的关系及意义。[方法]采用含20 000条Oligo DNA的人类全基因组寡核苷酸芯片,对5例维吾尔族宫颈癌与宫颈非病变组织成组检测差异表达基因,通过MAS分析得到两组间凋亡相关的差异表达基因:Bcl-2、Bcl-xl、c-IAP1,并采用RT-PCR及免疫组化验证芯片检测其m RNA和蛋白的表达。[结果]与宫颈非病变组织相比,维吾尔族宫颈癌患者中凋亡相关基因Bcl-2、Bcl-xl和c-IAP1 m RNA和蛋白表达均上调。[结论]基因芯片筛选出的肿瘤差异基因,对于研究新疆宫颈癌发生的分子机制及宫颈癌的临床早期诊疗具有重要意义,有利于宫颈癌的易感性预测、早期诊断及疾病监测。维吾尔族宫颈癌的发生与细胞增殖、凋亡的动态平衡密切相关,IAP家族和Bcl-2家族抗凋亡基因在维吾尔族宫颈癌组织中高表达可能在宫颈癌抗凋亡机制中起着重要作用。
[Objective] To investigate the relationship between the expression of Bcl-2, Bcl-xl and c-IAP1 in Uigur cervical cancer and cervical non-diseased tissues and to understand the relationship between Uighur cervical cancer and apoptosis genes. [Methods] Human genome-wide oligonucleotide microarray containing 20 000 Oligo DNAs was used to detect the differentially expressed genes in 5 Uighur cervical cancer patients and non-diseased cervical tissues. MAS analysis showed that the apoptosis-related The differentially expressed genes Bcl-2, Bcl-xl and c-IAP1 were detected by RT-PCR and immunohistochemistry. [Results] Compared with cervical non-diseased tissue, the expression of Bcl-2, Bcl-xl and c-IAP1 m RNA and protein in Uighur cervical cancer patients were up-regulated. [Conclusion] The genes differentially expressed by cDNA microarray are important for studying the molecular mechanism of cervical cancer in Xinjiang and the early clinical diagnosis and treatment of cervical cancer, which is benefit for the prediction of cervical cancer susceptibility, early diagnosis and disease monitoring. The occurrence of cervical cancer in Uighur is closely related to the dynamic balance of cell proliferation and apoptosis. The high expression of IAP family and anti-apoptotic gene of Bcl-2 family in Uighur cervical cancer may play an important role in the anti-apoptotic mechanism of cervical cancer .