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目的:研究白藜芦醇体内抑制肺癌瘤块生长活性以及其体内外抗氧化活性。方法:采用肺癌Lewis C57BL/6J小鼠模型评价白藜芦醇的抗肿瘤活性,并测定荷瘤小鼠血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;采用活性氧(ROS)荧光探针测定白藜芦醇对人非小细胞肺癌SPC-A-1细胞内的ROS含量,并测定培养液中的SOD活性和MDA含量。结果:白藜芦醇能显著抑制体内肺癌瘤块的生长,高、中、低剂量组的抑制率分别为(58.4±5.5)%、(46.9±17.4)%和(31.9±8.1)%。白藜芦醇能上调荷瘤小鼠和SPC-A-1细胞的SOD活性,并降低MDA含量,还降低肿瘤细胞内的ROS含量。结论:结果表明白藜芦醇能抑制体内肺癌瘤块的生长;其抗氧化作用是其抗肺癌的主要机制之一。
OBJECTIVE: To study the inhibitory effect of resveratrol on the growth of lung cancer nodules and its anti-oxidative activity in vitro and in vivo. Methods: The anti-tumor activity of resveratrol was evaluated by Lewis C57BL / 6J mouse lung cancer model. The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) ROS) fluorescent probe was used to determine the level of ROS in resveratrol-treated human non-small cell lung cancer SPC-A-1 cells. The activity of SOD and the content of MDA in the culture medium were determined. RESULT: Resveratrol significantly inhibited the growth of lung cancer mass in vivo. The inhibition rates of high, medium and low dose groups were (58.4 ± 5.5)%, (46.9 ± 17.4)% and (31.9 ± 8.1)%, respectively. Resveratrol could up-regulate the SOD activity of tumor-bearing mice and SPC-A-1 cells, decrease the content of MDA and decrease the content of ROS in tumor cells. CONCLUSIONS: Resveratrol can inhibit the growth of lung cancer mass in vivo, and its anti-oxidant effect is one of the main mechanisms of anti-lung cancer.