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目的探讨苯并(a)芘(BaP)对小鼠肝细胞凋亡及其与脂质过氧化的关系。方法ICR小鼠灌胃染毒,染毒剂量分别为0、5、10、20、40mg/kg,应用吖啶橙/溴化乙啶(A0/EB)荧光双染法检测肝细胞凋亡情况并测定肝组织脂质过氧化主要终产物丙二醛(MDA)的含量。结果BaP各染毒剂量组肝细胞凋亡发生率与对照组比较,差异均有统计学意义(P<0.05),MDA含量40mg/kgBaP染毒剂量组与对照组比,较差异具有统计学意义(P<0.05)。结论BaP可诱发小鼠肝细胞发生凋亡和脂质过氧化,氧化损伤参与了介导细胞凋亡的过程。
Objective To investigate the effect of BaP on hepatocyte apoptosis and its relationship with lipid peroxidation in mice. Methods ICR mice were intragastrically administered with the doses of 0, 5, 10, 20 and 40 mg / kg respectively. Apoptosis of hepatocytes was detected by acridine orange / ethidium bromide (A0 / EB) The contents of malondialdehyde (MDA), the major lipid peroxidation product in liver tissues, were determined. Results Compared with the control group, the incidence of hepatocellular apoptosis in BaP exposure groups was significantly different (P <0.05). Compared with the control group, the MDA content of 40 mg / kg BaP exposure group was significantly lower than that of the control group (P <0.05). Conclusion BaP can induce apoptosis and lipid peroxidation in hepatocytes of mice. Oxidative damage is involved in the process of cell apoptosis.