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以野生蝉花菌种为试材,采用液体培养法,研究了碳源、氮源、微量营养和pH对野生蝉花菌种生长的影响,以期为标准化、规模化、产业化人工栽培蝉花提供优质种源。结果表明:将野生蝉花菌株接种到液体培养基中,在19℃,转速120r·min-1恒温振荡摇床内培养,蝉花菌种的最适液体培养基组分为可溶性淀粉20g,奶粉10g,磷酸二氢钾0.5g,硫酸镁1g,维生素B1210片,水1 000mL,最适pH 5.0~6.0。
Using wild cicadas species as test material, the effects of carbon sources, nitrogen sources, micronutrients and pH on the growth of wild cicadae alba were studied by using liquid culture method, in order to standardize, scale and industrialized artificial cicadas Provide high-quality provenance. The results showed that the wild cicadae strain inoculated into the liquid medium, at 19 ℃, speed 120r · min-1 shaking shaker at constant temperature, the optimum liquid culture medium for cocci fungi 20g soluble starch, milk powder 0.5 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate, 1210 tablets of vitamin B and 1 000 mL of water, and the optimum pH is 5.0 to 6.0.