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目的研究调节性T细胞(Treg)、Th17细胞的相关细胞因子在实验性自身免疫性肌炎小鼠淋巴结中表达的作用及意义。方法雌性BALB/c小鼠12只,随机分为正常对照组和模型组,模型组采用肌球蛋白诱导实验性自身免疫性肌炎(EAM)。采用实时荧光定量PCR(qRT-PCR)检测各组小鼠淋巴结中与Treg和Th17细胞分化及功能相关的细胞因子Foxp3、IL-10、TGF-β、RORγt、IL-6、IL-17、IL-23的mRNA表达。结果与正常小鼠相比,肌炎组小鼠淋巴结中Treg的转录因子Foxp3表达降低,相关的细胞因子IL-10、TGF-βmRNA表达水平增高;Th17的转录因子RORγt及相关的细胞因子IL-6、IL-17、IL-23 mRNA表达有不同程度的升高(P<0.05)。结论实验性自身免疫性肌炎中Treg表达降低,而Th17及其相关因子表达升高,提示Treg和Th17之间的失衡在肌炎发病机制中起一定作用。
Objective To study the role and significance of the expression of regulatory cytokines (Treg) and Th17 cells in the lymph nodes of mice with experimental autoimmune myositis. Methods Twelve female BALB / c mice were randomly divided into normal control group and model group. Myosin induced experimental autoimmune myositis (EAM) in model group. The expression of Foxp3, IL-10, TGF-β, RORγt, IL-6, IL-17 and IL-8 in lymph nodes of Treg and Th17 cells in different groups were detected by qRT- -23 mRNA expression. Results Compared with normal mice, the expression of Foxp3, a transcription factor of Treg, was increased in lymph nodes in mice with myositis and the expression of related cytokines IL-10 and TGF-βmRNA was increased. The transcription factor RORγt of Th17 and related cytokines IL- 6, IL-17, IL-23 mRNA expression increased to some extent (P <0.05). Conclusion The expression of Treg in experimental autoimmune myositis decreased and the expression of Th17 and its related factors increased, suggesting that the imbalance between Treg and Th17 may play a role in the pathogenesis of myositis.