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目的探讨选择性环氧合酶(COX-2)抑制剂塞来昔布对大鼠颅脑创伤后B淋巴细胞瘤-2基因(Bcl-2)表达及学习记忆功能的影响。方法实验分为对照组、假手术组、脑创伤组、治疗组,首先采用Marmarou方法建立大鼠闭合性颅脑创伤模型,qPCR法检测mRNA表达量,免疫组织化学法检测蛋白表达水平,TUNEL染色法检测细胞凋亡,Morris水迷宫实验测试学习记忆功能。结果脑创伤组COX-2的表达明显高于其他3组(P<0.05),治疗组较脑创伤组能有效降低COX-2的表达(P<0.05);而脑创伤组Bcl-2的表达明显低于其他3组(P<0.05),治疗组与脑创伤组比较能有效增高Bcl-2的表达(P<0.05);脑创伤组TUNEL染色阳性细胞数均高于其它各组(P<0.05),治疗组相较于脑创伤组能显著降低阳性细胞数(P<0.05);脑创伤组搜索平台所需的时间大于其他3组(P<0.05),治疗组较脑创伤组能有效降低搜索平台所需时间(P<0.05)。结论塞来昔布通过对COX-2的特异性抑制,减轻颅脑创伤后的炎症反应,增加Bcl-2的表达,从而抑制细胞的凋亡,发挥脑保护作用,并改善其脑创伤后的学习记忆功能障碍。
Objective To investigate the effect of selective cyclooxygenase (COX-2) inhibitor celecoxib on the expression of Bcl-2 and the learning and memory function of Bcl-2 after traumatic brain injury in rats. Methods The experimental group was divided into control group, sham operation group, traumatic brain injury group and treatment group. Marcarou method was used to establish the model of closed traumatic brain injury in rats. QPCR method was used to detect the expression of mRNA. The expression of TUNEL protein was detected by immunohistochemistry. Apoptosis was measured by the method of Morris water maze test learning and memory function. Results The expression of COX-2 in traumatic brain injury group was significantly higher than that in other three groups (P <0.05), and the expression of COX-2 in traumatic brain injury group was lower than that in traumatic brain injury group (P <0.05) (P <0.05). Compared with the traumatic brain injury group, the expression of Bcl-2 in the traumatic brain injury group was significantly increased (P <0.05). The number of TUNEL positive cells in brain injury group was higher than that in other groups (P < 0.05). Compared with the traumatic brain injury group, the number of positive cells in the traumatic brain injury group was significantly decreased (P <0.05). The search time of traumatic brain injury group was longer than the other three groups (P <0.05), and the treatment group was more effective than the traumatic brain injury group Reduce the search platform time (P <0.05). Conclusion Celecoxib can reduce the inflammatory reaction after traumatic brain injury and increase the expression of Bcl-2 through the specific inhibition of COX-2, so as to inhibit the apoptosis of the cells and exert the neuroprotective effect and to improve the brain injury Learning and memory dysfunction.