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为研究三株益生菌发酵中药制剂对鲤鱼血清生化指标、肝脏抗氧化指标及肠道菌群结构的影响,选用初始体重为(11.0±1.0)g的鲤鱼360尾,分为4个组,每组30尾,设3个重复。Ⅰ组为对照组,饲喂基础日粮;Ⅱ、Ⅲ、Ⅳ组分别为产朊假丝酵母组、枯草芽孢杆菌组、鼠李糖乳杆菌发酵中药组。养殖56 d后,测定血清生化指标、肝脏抗氧化指标,并采用DGGE法测定肠道菌群结构。结果表明:Ⅱ组、Ⅲ组、Ⅳ组的LSZ、C3、CAT、SOD,Ⅲ组、Ⅳ组的T-AOC均显著高于Ⅰ组(P<0.05),MDA显著低于Ⅰ组(P<0.05)。DGGE分离图谱及聚类分析相似性范围为0.29~0.77,Ⅳ组与Ⅰ组、Ⅱ组、Ⅲ组的相似性较低;且Ⅲ组、Ⅳ组鲤鱼肠道菌群丰富度显著高于Ⅰ组、Ⅱ组(P<0.05)。研究表明,摄食益生菌中药发酵饲料对鲤鱼生化指标、抗氧化指标及肠道菌群结构均有明显影响。
In order to study the effect of three strains of probiotics on serum biochemical indexes, liver antioxidation indexes and intestinal microflora of three common carp strains, 360 carps with initial body weight of (11.0 ± 1.0) g were divided into 4 groups Group 30, set 3 repeat. Group Ⅰ as the control group, fed the basal diet; group Ⅱ, Ⅲ, Ⅳ were Candida utilis group, Bacillus subtilis group, Lactobacillus rhamnosus fermentation group. After 56 days of culture, serum biochemical indexes and liver anti-oxidation indexes were measured, and the intestinal flora structure was determined by DGGE method. The results showed that the T-AOC of LSZ, C3, CAT, SOD, Ⅲ and Ⅳ of group Ⅱ, Ⅲ and Ⅳ were significantly higher than that of group Ⅰ (P <0.05) 0.05). The similarity of DGGE and cluster analysis ranged from 0.29 to 0.77, the similarity between group Ⅳ and group Ⅰ, group Ⅱ and group Ⅲ was lower than that of group Ⅰ, and the abundance of intestinal flora in group Ⅲ and group Ⅳ was significantly higher than that of group Ⅰ , Group Ⅱ (P <0.05). Studies have shown that feeding probiotic Chinese fermented feed on carp biochemical indicators, antioxidant indicators and intestinal flora have a significant impact.