五十年代以来,在组织培养技术发展的基础上,许多学者开展了人成血细胞系(haematopoietic cell line)培养的研究。最先报告的是 Osgood 等从人的外周血和骨髓中分离出白细胞进行体外的长期培养,在急性和慢性白血病人外周血中分离的白细胞,有三株获得持续生长和增殖达6个月 Since the 1950s, many scholars have conducted studies on the culture of haematopoietic cell lines based on the development of tissue culture technology. The first report was that Osgood et al. Isolated white blood cells from human peripheral blood and bone marrow for long-term in vitro culture. Three white blood cells isolated from the peripheral blood of acute and chronic leukemia patients, and three of them achieved sustained growth and proliferation for 6 months