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目的探索对人致病的副溶血性弧菌的检测方法,评价5种检测方法对副溶血性孤菌致病性测定的有效性。方法采用耐热溶血毒素tdh基因和耐热直接溶血毒素相关毒素trh基因实时荧光定量PCR检测、并与神奈川溶血试验、小鼠腹腔注射和灌胃法对比,分别检测分离自腹泻者和自然环境中副溶血性弧菌标本。结果两类来源的副溶血性弧菌在tdh基因的检出率具有统计学意义(χ2=169.5,P<0.01),在神奈川溶血试验中差异有统计学意义(χ2=29.3,P<0.01),在trh基因、小鼠腹腔注射以及灌胃检测无统计学意义。结论 tdh基因实时荧光定量PCR法是快速筛查致病性副溶血性弧菌的最好方法。
Objective To explore the detection method of human pathogenic Vibrio parahaemolyticus and to evaluate the effectiveness of the five detection methods on the pathogenicity of Vibrio parahaemolyticus. Methods The tdh gene of heat-resistant toxin and heat-resistant direct hemolysin-related toxin trh gene were detected by real-time fluorescence quantitative PCR and compared with Kanagawa hemolysis test, intraperitoneal injection and gavage in mice, respectively. Vibrio parahaemolyticus specimens. Results The detection rate of tdh gene in two types of Vibrio parahaemolyticus was statistically significant (χ2 = 169.5, P <0.01), and the difference was statistically significant in the Kanagawa hemolysis test (χ2 = 29.3, P <0.01) , In the trh gene, intraperitoneal injection of mice and gavage test was not statistically significant. Conclusion tdh gene real-time fluorescence quantitative PCR method is the best method for rapid screening of pathogenic Vibrio parahaemolyticus.