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为了解济南市流行性感冒(流感)的流行规律及病毒亚型变化情况,及为进一步做好流感预防控制工作提供科学有效的防治依据,对济南市2010-2013年流感网络监测系统的资料进行整理和分析,资料内容包括人口学特征、就诊情况及病原监测结果等信息。采用实时荧光RT-PCR方法检测流感病毒核酸;采用MDCK细胞对样本进行病毒分离。结果显示:2010年5月至2013年9月,济南市2所哨点医院共报告流感样病例数8 732例,占门诊发热病例的1.19%(8 732/732 174)。济南市共检测2 644例流感样病例咽拭子样本,其中阳性338例,阳性率为12.78%。在338例阳性中,甲型H1N1型113例,占33.43%;H3N2型80例,占23.67%;B型145例,占42.90%。2013年济南市H3N2亚型流感病毒分离株与WHO推荐疫苗株核苷酸(氨基酸)同源性为97.8%~98.6%,99.3%~99.7%。济南市2010-2011年流感流行季节中甲型H1N1、H3N2、B型三型均有出现、2011-2012年流感优势毒株以H3N2、B型为主;2012-2013年流感优势毒株以甲型H1N1为主。
In order to understand the prevalence of influenza (influenza) and the changes of virus subtypes in Jinan and to provide a scientific and effective basis for further prevention and control of influenza, the data of influenza network monitoring system in Jinan City from 2010 to 2013 Collation and analysis, information content includes demographic characteristics, treatment and pathogen monitoring results and other information. Real-time fluorescence RT-PCR method was used to detect influenza virus nucleic acid; MDCK cells were used for virus isolation. The results showed that from May 2010 to September 2013, a total of 8 732 cases of influenza-like illness were reported in 2 sentinel hospitals in Jinan, accounting for 1.19% (8 732/732 174) of out-patient fever cases. Jinan City, a total of 2 644 cases of flu-like throat swab samples were detected, of which 338 were positive, the positive rate was 12.78%. Among the 338 positive cases, 113 cases were type A H1N1, accounting for 33.43%; 80 cases were H3N2 type accounting for 23.67%; 145 cases were type B, accounting for 42.90%. In 2013, the nucleotide (amino acid) homology between the H3N2 influenza virus isolates from Jinan and the WHO recommended vaccine strains was 97.8% -98.6% and 99.3% -99.7%, respectively. Influenza A H1N1, H3N2 and B type were all found in Jinan City during the 2010-2011 influenza season. H3N2 and B predominant influenza strains were present in 2011-2012. In 2012-2013, H1N1-based.