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为探讨亚麻种间杂交不易亲和的原因并采取相应有效的克服措施,本试验以栽培亚麻和野生亚麻为材料,采用单次授粉、重复授粉和重复授粉+生长调节剂3种杂交技术,通过胚珠整体染色与透明法,结合微分干涉衬显微镜观察,对栽培亚麻×野生亚麻杂交授粉后胚乳及胚的发育进行了较为系统的研究。结果显示:单次授粉多数胚囊内未形成正常的胚乳及胚,重复授粉和重复授粉+生长调节剂处理后胚囊内可形成胚乳及胚,但发育进程比对照(栽培亚麻自交)较为滞后。用生长调节剂处理子房,能刺激子房膨大,延缓子房脱落,有利于形成杂交种子。由此可见,重复授粉+生长调节剂处理是克服亚麻种间杂交不亲和性的有效措施。
In order to explore the reasons for the incompatibility of the interspecific hybridization of flax and to take appropriate and effective measures to overcome them, the experiments were conducted on the cultivated flax and wild flax using three kinds of hybridization techniques: single pollination, repeated pollination and repeated pollination + growth regulator, Ovum whole staining and transparent method, combined with differential interference microscopy microscopy, cultivated flax × wild flax hybridization after pollination of endosperm and embryo development more systematic study. The results showed that the endosperm and embryos were not formed in most embryo sacs of single pollination, and the endosperm and embryos were formed in the embryo sacs after repeated pollination and repeated pollination plus growth regulators, but the developmental process was more than that of the control Lag Treatment of ovarian growth regulators, can stimulate ovarian enlargement, delayed ovary off, is conducive to the formation of hybrid seeds. Thus, repeated pollination + growth regulator treatment is to overcome the flax hybridization between the effective measures.