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[目的]观察雷帕霉素(RAPA)对肝纤维化模型大鼠肝组织内基质金属蛋白MMP-2,MMP-13表达水平的影响,探讨RAPA抑制大鼠肝纤维化的作用机制.[方法]将实验大鼠随机分为正常对照组、模型对照组及RAPA干预组.以四氯化碳花生油构建大鼠肝纤维化模型,RAPA干预组于实验第6周灌胃给予2mg/kg RAPA,每日1次,持续2周.应用光学显微镜观察肝纤维化情况,免疫组织化学方法观察模型对照组和RAPA干预组MMP-2,MMP-13蛋白表达水平.[结果]常规染色切片观察结果显示,模型对照组肝组织呈混合性脂肪变性,细胞体积明显增大,纤维间隔明显增生,成纤维细胞及炎症细胞增生浸润;RAPA干预组肝纤维化程度较模型对照组明显减轻,组织结构清晰.免疫组织化学观察结果显示,与模型对照组比较,RAPA干预组MMP-13蛋白表达明显增高(P<0.05),而MMP-2蛋白表达明显下降(P<0.05).[结论]RAPA通过上调肝组织中MMP-13表达及下调MMP-2表达从而缓解肝纤维化进展.
[Objective] To observe the effect of Rapamycin (RAPA) on the expression of matrix metalloproteinase-2 and matrix metalloproteinase-13 (MMP-2, MMP-13) in liver tissue of rats with liver fibrosis and to explore the mechanism of RAPA inhibiting hepatic fibrosis in rats. ] The experimental rats were randomly divided into normal control group, model control group and RAPA intervention group.The rat hepatic fibrosis model was established by carbon tetrachloride peanut oil.The RAPA intervention group was given 2mg / kg RAPA at the 6th week of experiment, Once a day for 2 weeks. The liver fibrosis was observed by optical microscopy and the expression of MMP-2 and MMP-13 protein in model control group and RAPA intervention group were observed by immunohistochemical method. [Results] The results of routine staining sections The model control group showed mixed steatosis, increased cell volume, obvious fiber interstitial hyperplasia, fibroblasts and inflammatory cells proliferation and infiltration. Compared with the model control group, the degree of hepatic fibrosis in RAPA intervention group was significantly reduced and the tissue structure was clear. The results of immunohistochemistry showed that compared with the model control group, the expression of MMP-13 protein in RAPA group was significantly increased (P <0.05), while the expression of MMP-2 protein was significantly decreased (P <0.05). [Conclusion] The expression of MMP-13 in tissues and tissues Down-regulate the expression of MMP-2 to alleviate the progress of liver fibrosis.