论文部分内容阅读
对小鼠艾氏腹水癌细胞及人直肠粘液腺癌在深低温（－１９６℃）冷冻前后的功能和超微结构进行了比较研究；结果表明：与对照组相比，两种癌细胞的ＦＤＡ荧光强度和￣３Ｈ－ＴｄＲ掺入率显著降低（Ｐ＜０．０１）．乳酸脱氢同工酶的Ｍ亚基显著增加，Ｈ亚基明显减少，Ｍ／Ｈ比值提高。电镜观察到癌细胞内和核内形成空泡，胞质凝缩，线粒体膨胀，核膜破裂，核质外流，染色质凝集呈块状不均匀分布等超微结构变化；说明深低温冷冻对肿瘤细胞的膜完整性、ＤＮＡ合成功能、酶活性及超微结构等均有显著的损伤作用。
The function and ultrastructure of mouse Ehrlich ascites carcinoma cells and human rectal mucinous adenocarcinoma before and after deep cryogenic (-196°C) freezing were compared; the results showed that: Compared with the control group, the FDA of two kinds of cancer cells The fluorescence intensity and ̄3H-TdR incorporation rate were significantly decreased (P<0.01). The M subunits of lactate dehydrogenase isoenzymes increased significantly, the H subunits decreased significantly, and the M/H ratio increased. Electron microscopy revealed that vacuoles were formed inside and inside the cancer cells, cytoplasm condense, mitochondria swelled, nuclear membrane ruptured, nucleoplasm outflow, chromatin agglutination, and heterogeneous ultrastructural changes such as massive aggregates. The cell membrane integrity, DNA synthesis function, enzyme activity and ultrastructure all have significant damage effects.