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用活化的人Ｂ细胞株３Ｄ５细胞免疫ＢＡＬＢ／ｃ小鼠，取小鼠脾脏细胞与ＳＰ２／０细胞融合。融合后细胞置甲基纤维素半固体培养基生长。以０．５％甲醛处理的３Ｄ５细胞和ＣＥＭ细胞包被酶标板作为靶细胞，用细胞酶联免疫吸附（ＣＥＬＩＳＡ）试验筛选２９０个杂交瘤细胞克隆，得到３３个与３Ｄ５细胞反应阳性而与ＣＥＭ细胞反应阴性的克隆。再经间接免疫荧光染色后，用流式细胞仪复测以上所得３３个阳性克隆，结果３Ｄ５阳性ＣＥＭ阴性者２７例，占８１．８２％，表明ＣＥＬＩＳＡ法是一个粗筛抗人Ｂ细胞分化抗原的简便有效方法。
BALB/c mice were immunized with activated human B cell line 3D5 cells, and mouse spleen cells were fused with SP2/0 cells. After fusion, the cells were grown on methylcellulose semi-solid medium. The 0.5% formaldehyde-treated 3D5 cells and CEM cells were coated with the enzyme-labeled plates as target cells, and 290 hybridoma cell clones were screened using a cell-enzyme-linked immunosorbent assay (CELISA) assay, resulting in 33 positive reactions with 3D5 cells. CEM cell-negative clones. After indirect immunofluorescence staining, the 33 positive clones obtained above were retested by flow cytometry. Results showed that 27% of 3D5-positive CEM-negative individuals accounted for 81.82%, indicating that CELISA is a rough screening anti-human B cell differentiation antigen. Easy and effective method.