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血脑屏障(blood-brain barrier,BBB)是位于中枢神经系统(central nervous system,CNS)和中枢系统环境间的一层生理保护屏障.凡是作用于CNS的药物,必须先通过BBB.为了寻找能够进入CNS的药物,通过细胞培养时间优化和跨膜电阻测定等,建立了ECV304/C6共培养通过BBB药物筛选模型.并将该模型应用于从传统中药淫羊藿的提取物中,筛选可能作用于CNS的活性成分,结合高压液相色谱-质谱联用技术(HPLC-MS),对筛选出的化合物进行鉴定分析.研究结果表明,淫羊藿提取物中至少有13种成分能够穿越BBB模型,其中2种成分被确认为淫羊藿苷和宝藿苷Ⅰ,为CNS药物开发的早期快速筛选提供了实验依据.
The blood-brain barrier (BBB) is a layer of physiologic protective barrier located between the central nervous system (CNS) and the central nervous system, and any drug that acts on the CNS must first pass the BBB. Enter the CNS drug through the cell culture time optimization and transmembrane resistance determination, established the ECV304 / C6 co-culture through the BBB drug screening model and the model applied to the traditional Chinese medicine Epimedium extract, screening possible role The active compounds in CNS were identified and analyzed by HPLC-MS.The results showed that at least 13 components of Epimedium brevicornum extract could cross BBB model , Of which two components were identified as icariin and treasure glycosides Ⅰ, for the rapid development of CNS drug provides an experimental basis for rapid screening.