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通过耗竭蛋白激酶Ｃ（ＰＫＣ），用ＰＫＣ抑制剂和激活剂等方法，观察ＰＫＣ在鼻咽癌细胞系ＣＮＥ－２Ｚ生长中的作用。发现：随ＴＰＡ浓度增大，细胞ＰＫＣ含量降低，生长指数也逐渐减少；作用于ＰＫＣ蛋白催化区的ＰＫＣ抑制剂Ｓｔａｕｒｏｓｐｏｒｉｎｅ（ＳＴ）与作用于调节区的抑制剂Ｓｐｈｉｎｇｏｓｉｎｅ（ＳＳ）均抑制ＣＮＥ－２Ｚ细胞的生长，最低有效浓度分别为２×１０－７ｍｏｌ／Ｌ与１０－５ｍｏｌ／Ｌ，随浓度增大而作用增强；ＰＫＣ刺激剂ＯＡＧ（０．１～１００μｇ／ｍｌ）单独作用时对ＣＮＥ－２Ｚ的生长无明显影响，但４μｇ／ｍｌ可基本阻断ＳＴ或ＳＳ最低有效浓度的抑制效应。提示：ＰＫＣ在鼻咽癌细胞ＣＮＥ－２Ｚ生长中起重要作用，阻断ＰＫＣ的作用将为鼻咽癌的防治提供新的途径。
The role of PKC in the growth of nasopharyngeal carcinoma cell line CNE-2Z was observed by depleting protein kinase C (PKC), using PKC inhibitors and activators. The results showed that with the increase of TPA concentration, the content of PKC decreased and the growth index decreased gradually. Both PKC inhibitor Staurosporine (ST) and Sphingosine (SS) acting on the regulatory region of PKC protein inhibited CNE-2Z Cell growth, the minimum effective concentration of 2 × 10-7mol / L and 10-5mol / L, respectively, with increasing concentration and enhance the role; PKC stimulator OAG (0.1 ~ 100μg / ml) 2Z had no significant effect on the growth, but 4μg / ml could basically block the inhibitory effect of ST or SS minimum effective concentration. Tip: PKC plays an important role in the growth of nasopharyngeal carcinoma cells CNE-2Z, blocking the role of PKC will provide a new way for the prevention and treatment of nasopharyngeal carcinoma.