经C_(57)BL/6小鼠脾内注射同系B_(16)—黑色素瘤细胞,建立实验性肝转移模型。经脾脏(腹外法)直接注射0.1ml瘤细胞悬液(含2×10~6个瘤细胞),肿瘤细胞经脾静脉进入门静脉而停驻于肝脏,18d后肝转移瘤发生率为70%。与经典的大容积脾内注射法(注射量为1ml瘤细胞悬液,1min后切脾)相比,本法保留了脾脏,有更好的临床相关性,从而提供了一种良好的实验性肝转移模型。 C_(57)BL/6 mice were injected intraperitoneally with syngeneic B_(16)-melanoma cells to establish an experimental hepatic metastasis model. The spleen (abdominal method) directly injected 0.1 ml of tumor cell suspension (containing 2×10~6 tumor cells). Tumor cells entered the portal vein via the splenic vein and stopped in the liver. The incidence of hepatic metastases was 70% after 18 days. . Compared with the classic large-volume splenic injection method (injection of 1 ml of tumor cell suspension and splenectomy of 1 min later), this method retains the spleen and has better clinical relevance, thus providing a good experimental Liver metastasis model.