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【目的】利用核糖体DNA联合序列探讨天牛总科高阶元分子系统发育。【方法】本研究采用分子标记技术,分析测定了63种天牛核糖体28S rDNA D2和D3区以及18S rDNA V4和V7区的DNA序列,并采用邻接法、最大似然法和贝叶斯推论法分别构建了天牛总科2科6亚科63种的分子进化系统。【结果】序列联合比对分析,最终得到1 404 bp的联合数据组,其中可变位点446个(32.0%),保守位点958(68.0%),转换/颠换的平均值(R值)为1.73。28S rDNA和18S rDNA以及联合序列的饱和度分析显示碱基突变未达到饱和,说明这些序列适合于分子进化树的构建。利用不同系统发育重建方法得到进化树具有相似拓扑结构,结果支持沟胫天牛亚科、花天牛亚科和天牛亚科为单系群,这与形态学分类结果相似;狭胸天牛独立成为亚科得到了支持。【结论】利用28S rDNA D2和D3区以及18S rDNA V4和V7区联合序列成功构建出了天牛总科高阶元的系统发育树。研究表明联合序列分析是探讨天牛高阶元分类的有效的方法。
【Aim】 To investigate the phylogeny of higher-order metabonomics in the genus Buforacea by using ribosomal DNA sequence. 【Method】 In this study, the DNA sequences of 28S rDNA D2 and D3 and 18S rDNA V4 and V7 regions of 63 species of Beet were determined by molecular marker technique. Adjacent method, maximum likelihood method and Bayesian inference Act respectively, the construction of the cephalopods 2 subfamilies 6 Subfamilies 63 kinds of molecular evolution system. 【Result】 The results of sequence alignment analysis showed that 1 404 bp combined data set was obtained, including 446 variable sites (32.0%), 958 (68.0%) conserved sites, the average value of transformation / transversion ) Of 1.73.28S rDNA and 18S rDNA as well as the saturation of the combined sequences showed that the base mutations did not reach saturation, indicating that these sequences are suitable for the construction of molecular phylogenetic tree. Phylogenetic tree is similar to the topological structure of phylogenetic tree. The results support that the genus Glyptostroboides, Celastrus yunnanensis and Tetranychidae are monophyletic groups, which is similar to morphological classification; stand by. 【Conclusion】 The phylogenetic tree of higher-order metazoan cephalopods was successfully constructed using 28S rDNA D2 and D3 regions and 18S rDNA V4 and V7 regions. Studies have shown that the joint sequence analysis is an effective method to explore the higher-order metabonomics.