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以乙二醛、乙二胺和尿素为原料，通过Ｍａｎｎｉｃｈ反应，制得了２，５，７，９－四氮杂双环［４，３．０］壬酮－８二盐酸盐一水合物。
Using glyoxal, ethylenediamine and urea as raw materials, 2,5,7,9-tetraazabicyclo [4,3.0] nonanone-8 dihydrochloride monohydrate was obtained by Mannich reaction.