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目的研究芦荟苷(Barbaloin,Barb)对急性心肌缺血再灌注损伤大鼠心肌细胞凋亡的影响。方法结扎大鼠左冠状动脉前降支复制心肌缺血再灌注损伤模型,实验分为假手术组、缺血再灌注组(IR)、Barb高、低剂量预处理组。采用原位缺口末端标记法(TUNEL)检测心肌细胞凋亡指数(AI),ELISA法测定血清TNF-α水平,化学法测定线粒体Ca2+-ATPase活性。结果与IR组比较,Barb组AI和TNF-α水平显著降低(P<0.05或P<0.01),Ca2+-ATPase活性明显增高(P<0.05)。结论Barb能明显抑制IR引起的心肌细胞凋亡,其作用可能与降低TNF-α水平和提高Ca2+-ATPase活性有关。
Objective To study the effect of Barbaloin (Barb) on cardiomyocyte apoptosis in rats with acute myocardial ischemia-reperfusion injury. METHODS: The left anterior descending coronary artery was ligated to replicate myocardial ischemia-reperfusion injury model in rats. The experiment was divided into sham operation group, ischemia reperfusion group (IR), Barb high and low dose pretreatment groups. Apoptosis index (AI) of cardiomyocytes was detected by in situ nick end labeling (TUNEL), serum TNF-α levels were determined by ELISA, and mitochondrial Ca2+-ATPase activity was measured by chemical method. Results Compared with IR group, the levels of AI and TNF-α in Barb group were significantly decreased (P<0.05 or P<0.01), and the activity of Ca2+-ATPase was significantly higher (P<0.05). Conclusion Barb can significantly inhibit IR-induced cardiomyocyte apoptosis, which may be related to the decrease of TNF-α and Ca2+-ATPase activity.