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作者应用对流免疫电泳(OEP)等方法对小鼠、大鼠和兔的肝片吸虫病进行了感染后和治疗前后的血清学检测的研究,并报告了采用凝胶柱层析法分离提纯抗原的结果。实验所用抗原,以肝片吸虫成虫研成匀浆,制成冰冻干粉。取10mg冻干粉加入3mlpH7.10.01MPBS,于冰浴中研磨后,经离心沉淀,上清波即为FhWWE粗抗原,用于CEP。免疫双扩散试验(ID),也用此类抗原,但以40mg冻干粉加1ml的PBS制成。纯化抗原系将FhWWE用Sephacryl S-200凝胶柱层析法,取得各组分,经试验,取第Ⅲ、Ⅳ两峰的洗脱液,经浓缩、透析,制成含蛋白量约为1. 8mg/ml的纯化抗原。CEP:采用琼脂糖,巴比妥-盐酸缓冲系统。检测小鼠和兔血清时选用1:300(W/V)的FhWWE;对大鼠血清则用纯化抗原。ID:
The authors applied the method of convective immuno-electrophoresis (OEP) to study the serological detection of hepatic snipositis in mice, rats and rabbits before and after infection, and reported the separation and purification of antigen by gel column chromatography the result of. Antigens used in the experiment, with liver Fasciola adult research into homogenized, made of freeze-dried powder. Take 10mg freeze-dried powder was added 3mlpH7.10.01MPBS, after grinding in an ice bath, by centrifugation, the upper wave was FhWWE crude antigen for CEP. Immune double diffusion test (ID), but also with such antigens, but with 40mg lyophilized powder plus 1ml of PBS made. Purified antigens will FhWWE by Sephacryl S-200 gel column chromatography to obtain the components, the test, take the first Ⅲ, Ⅳ peak eluent, after concentration, dialysis, made with a protein content of about 1 Purified antigen at 8 mg / ml. CEP: using agarose, barbiturate - hydrochloric acid buffer system. 1: 300 (W / V) FhWWE was used for the detection of mouse and rabbit serum; purified antigen was used for the rat serum. ID: