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除血液外,骨是最常用的移植组织,但植入前骨的污染不可避免,从而引起手术伤口的感染和移植失败。许多学者推荐了多种处理污染移植骨的方法,本文作者对各种方法进行了评价。 材料知方法 取5只成年Sprague-Dawley鼠的股骨,切成8mm×3.5mm大小的骨块,均放入盛有10ml绿脓杆菌(Pseudomonas aeroginosa)和10ml金黄色葡萄球菌(Staphylococcus aurus)混浊液中45s,而后将5类骨块分成8组,分别进行以下处理:①高压蒸气灭菌11min;②放入30ml10％聚乙烯吡咯烷酮—碘溶液(povidone-iodine)中15min;③放入30ml4％葡萄糖洗必太和4％乙醇溶液中15min;①放入30mlNeosporin液(含40mg新霉素和多粘菌素B200000 U/
In addition to blood, bone is the most commonly used transplanted tissue, but contamination of the anterior bones is unavoidable, causing infection and graft failure in surgical wounds. Many scholars recommend a variety of methods of treating contaminated bone graft, and the authors evaluate various methods. Materials Method Five femoras of adult Sprague-Dawley rats were cut into 8 mm x 3.5 mm bone fragments and placed in a suspension containing 10 ml of Pseudomonas aeroginosa and 10 ml of a suspension of Staphylococcus aurus In 45s, and then divided into five groups of five types of bone were treated as follows: ① high-pressure steam sterilization 11min; ② into 30ml10% polyvinylpyrrolidone-iodine solution (povidone-iodine) for 15min; ③ Add 30ml4% glucose Wash mustard and 4% ethanol solution 15min; ① into 30mlNeosporin solution (containing 40mg neomycin and polymyxin B200000 U /