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目的 :观察新型氨基甾体 (KH)对小鼠粒单系白血病细胞系WEHI 3B细胞增殖、分化和凋亡的影响 ,并初步探讨其作用机制。方法 :采用集落培养及细胞毒性实验检测KH对WEHI 3B细胞增殖的影响 ;用NBT还原实验及NSE染色检测KH对WEHI 3B细胞分化的影响 ;用形态学观察和DNA片段凝胶电泳检测KH对WEHI 3B细胞凋亡的影响 ;用RT PCR检测癌基因c mycmRNA在WEHI 3B细胞中表达水平的变化。结果 :KH(10 -8～ 10 -4 mol·L-1)能抑制WEHI 3B细胞增殖 ;其较低浓度 (10 -8～ 10 -6mol·L-1)即可诱导WEHI 3B细胞向单核巨噬样细胞分化 ,较高浓度 (10 -6～ 10 -4 mol·L-1)诱导WEHI 3B细胞的凋亡作用增强 ;KH在抑制WEHI 3B细胞增殖 ,诱导其分化和凋亡过程中癌基因c mycmRNA表达水平明显降低。结论 :KH能抑制WEHI 3B细胞增殖 ,诱导其分化和凋亡。上述作用与癌基因c myc的表达有关。
OBJECTIVE: To observe the effects of novel aminosteroids (KH) on the proliferation, differentiation and apoptosis of mouse monoblastic leukemia cell line WEHI 3B and to explore its mechanism. Methods: The effects of KH on the proliferation of WEHI 3B cells were detected by colony culture and cytotoxicity assay. The effects of KH on the differentiation of WEHI 3B cells were detected by NBT reduction assay and NSE staining. The morphological changes and DNA fragment gel electrophoresis were used to detect the effects of KH on WEHI 3B cell apoptosis. RT-PCR was used to detect the expression of oncogene c mycmRNA in WEHI 3B cells. RESULTS: KH (10 -8 ~ 10 -4 mol·L -1) inhibited the proliferation of WEHI 3B cells. When treated with 10 -8 ~ 10 -6 mol·L -1, WEHI 3B cells could be induced to mononuclear The macrophage-like cells differentiated and the apoptosis of WEHI 3B cells was enhanced at a higher concentration (10 -6 ~ 10 -4 mol·L -1). KH inhibited the proliferation of WEHI 3B cells and induced the differentiation and apoptosis of WEHI 3B cells Gene c mycmRNA expression was significantly reduced. Conclusion: KH can inhibit WEHI 3B cell proliferation and induce its differentiation and apoptosis. The above role and oncogene c myc expression.