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目的对广西1例HIV-1 G亚型毒株感染者体内病毒近似全长基因组进行序列测定并进行系统进化分析。方法对前期鉴定的1例HIV-1G亚型毒株感染者进行背景信息收集,并采集其外周血,提取病毒RNA,利用逆转录巢式-PCR技术分两段扩增并测定病毒近似全长基因序列。利用MEGA6软件对全长序列进行系统进化分析。结果获得了病毒近似全长基因组序列,长度为8847 bp。在系统进化树中,该毒株与G亚型参考株成簇,Bootstrap值为100%。系统溯源分析显示,该毒株与广西首次报道的G亚型JN106043毒株关系最近(Bootstrap值为100%,基因离散率为5%)。结论该毒株可能是由广西毒株传播所致,提示G亚型毒株在广西地区可能存在一定的局部流行,需进一步监测。该毒株序列的测定和分析对于广西地区HIV流行状况分析具有重要意义。
OBJECTIVE: To determine the sequence of the full-length genome of a virus infected by a HIV-1 G subtype in Guangxi and analyze its phylogenetic relationship systematically. Methods One case of HIV-1G subtype identified in the previous period was collected for background information, peripheral blood was collected and viral RNA was extracted. The virus was amplified in two stages by reverse transcription nested-PCR and the approximate full-length virus gene sequence. Phylogenetic analysis of full-length sequences using MEGA6 software. Results The full-length genome sequence of the virus was obtained, with a length of 8847 bp. In the phylogenetic tree, the strain was clustered with the G subtype reference strain with a Bootstrap value of 100%. The system traceability analysis showed that the strain was the closest to the G subtype JN106043 strain reported in Guangxi (the Bootstrap value was 100% and the genetic divergence rate was 5%). Conclusion The strain may be caused by the spread of Guangxi strains, suggesting that there may be some local epidemics of G subtype strains in Guangxi and need further monitoring. The determination and analysis of the strain’s sequence is of great significance for the analysis of HIV prevalence in Guangxi.