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根据风险=危险×暴露的原理,在实验室条件下评价转基因作物对非靶标节肢动物影响时,所选择的代表性非靶标生物通常是在农田系统中较高地暴露于转基因外源杀虫蛋白的节肢动物种.为了弄清Bt稻田主要节肢动物暴露于Cry蛋白的程度,选择合适的非靶标节肢动物,用于转基因抗虫水稻的风险评价,本文采用酶联免疫技术检测了水稻不同生长期从转cry2Aa基因水稻田采集的不同节肢动物体内Cry2Aa蛋白的含量.结果表明:不同节肢动物种体内的Cry蛋白含量差异显著.一些节肢动物体内不含Cry蛋白,而一些节肢动物体内含有较高的Cry蛋白;相对于花期后采集的节肢动物,在Bt水稻花期采集的节肢动物,特别是捕食性节肢动物体内的Cry蛋白含量较高;寄生性节肢动物体内未检测到Cry蛋白.这为在实验室条件下评价转基因水稻对农田非靶标节肢动物的影响奠定了基础.
Based on the principle of risk = risk × exposure, when evaluating the effects of GM crops on non-target arthropods under laboratory conditions, the representative non-target organisms selected are typically those exposed higher to the transgenic exogenous pesticidal protein in cropland systems Arthropod species.In order to understand the degree of exposure of Cry3Ab to arthropods in Bt paddy field and select appropriate non-target arthropods for risk assessment of transgenic insect-resistant rice, we used enzyme-linked immunosorbent assay Cry2Aa protein content in different arthropods collected from cry2Aa transgenic rice field showed that there were significant differences in Cry protein content in different arthropod species.Some arthropods did not contain Cry protein while some arthropods contained higher Cry Compared with the arthropods collected after flowering, the content of Cry protein in arthropods, especially predacious arthropods collected in the flowering stage of Bt rice was high, but Cry protein was not detected in the parasitic arthropods. Under the conditions of transgenic rice on non-target farmland arthropods laid the foundation.