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在我国冀西北高寒干旱,自然环境因素和栽培条件较差的生态区域内,研究了利用孤雌生殖诱导马铃薯双单倍体的技术,两年试验表明,诱导频率高于世界诱导频率最高的捷克(13.8~70.2株/100个浆果),较国际上广泛应用的高频率诱导者IVP35和IVP48高出30.9株/100个浆果,筛选出了配合力好,产生孤雌生殖能力强的四倍体品种中薯2号、79-6-19和CIP800935,诱导频率为11.1~33.3株/100个浆果。本项研究的创新点是实现了野生种优良基因向栽培种成功的导入;缩短了与国外同研究领域的差距,为加速河北省马铃薯种质资源创新及利用倍性水平合成育种提供了有效的方法。
In the ecologically-intensive area with cold and drought in northwestern Hebei, natural environmental factors and poor cultivation conditions, the technique of partial double haploid induction by parthenogenesis was studied. Two years of experiments showed that the induction frequency was higher than that of the highest inducible frequency in the world (13.8 ~ 70.2 plants / 100 berries), which was 30.9 higher than that of the IVP35 and IVP48 widely used in the world. Strong tetraploid varieties potato 2, 79-6-19 and CIP800935, the induction frequency of 11.1 to 33.3 plants / 100 berries. The innovative point of this research is to realize the successful introduction of the excellent genes of wild species into cultivated species; to shorten the gap with foreign research fields, and to provide an effective method for accelerating the innovation of potato germplasm resources and using the ploidy level of synthetic breeding in Hebei Province method.