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目的明确人类微小RNA196a(hsa-mi R-196a)在早期胃癌中异常表达模式,探讨其异常表达在早期胃癌发生与发展过程中的生物学作用。方法收集作者医院57例临床标本的包括52例早期胃癌患者手术及全血标本和5例非癌患者全血标本,以混合的正常人全血标本为对照,采用实时定量聚合酶链反应(polymerase chain reaction,PCR)检测hsa-mi R-196a在早期胃癌患者中的表达水平。利用生物信息学数据库预测hsa-mi R-196a调控的靶基因及信号通路。结果在52例早期胃癌标本中,44例(44/52,84.6%)手术标本高表达hsa-mi R-196a;42例(42/52,80.8%)全血标本高表达hsa-mi R-196a。利用生物信息学方法获得了211个可能受hsa-mi R-196a所调控的靶基因,这些靶基因参与了多条肿瘤相关信号通路。结论人类微小RNA196a在早期胃癌中高表达,这一表达模式可能通调控多条肿瘤相关信号通路参与胃癌的早期进展。
OBJECTIVE: To determine the abnormal expression pattern of hsa-mi R-196a in early gastric cancer and to explore the biological role of hsa-mi R196a in the development and progression of early gastric cancer. Methods Fifty-five patients with early gastric cancer were collected from surgical and whole blood samples and five non-cancer blood samples collected from 57 clinical samples of the author’s hospital. Using the mixed whole blood samples of normal people as control, real-time quantitative polymerase chain reaction chain reaction, PCR) was used to detect the expression of hsa-mi R-196a in patients with early gastric cancer. Using bioinformatics database to predict target genes and signal pathways regulated by hsa-mi R-196a. Results In the 52 specimens of early gastric cancer, hsa-mi R-196a was highly expressed in 44 (44/52, 84.6%) surgical specimens of 42 cases (42/52, 80.8% 196a. Totally 211 target genes regulated by hsa-mi R-196a were obtained by bioinformatics methods. These target genes are involved in several tumor-related signaling pathways. Conclusion Human microRNA196a is highly expressed in early gastric cancer. This expression pattern may be involved in the regulation of multiple tumor-related signaling pathways in the early progression of gastric cancer.