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[目的]分析早期和晚期肺腺癌的差异基因和信号通路。[方法]从美国国立生物信息中心(NCBI)的GEO数据库下载GSE10072数据集,去除临床指标缺失的样本,按照TNM分期将肺腺癌样本分为早期(Ⅰ期,共16例)和晚期(Ⅲ~Ⅳ期,共15例)两组。原始数据经dChip进行质量检验、标准化,然后进行差异基因分析。从MsigDB数据库获得344个生物信号基因集,通过GSEA进行信号通路富集分析。[结果]获得SEMA3、PLAU、CDKN2A等14个明显差异基因,获取的差异基因主要与细胞凋亡、细胞黏附等过程密切相关。选取MsigDB中来源于Bicarta、KEGG、GenMAPP三大数据库的344个基因集进行富集分析,结果发现Death pathway、Leukocyte transendothelial migration和Focaladhesion等22条信号通路在晚期肺腺癌中明显富集,富集通路主要涉及细胞凋亡、细胞黏附和迁移等过程。[结论]早期与晚期肺癌中存在一些明显的差异表达基因,有部分信号通路在晚期肺腺癌中明显富集。
[Objective] To analyze the differential genes and signal pathways of early and late lung adenocarcinoma. [Methods] The GSE10072 dataset was downloaded from the GEO database of the National Center for Biological Information (NCBI) to remove samples lacking clinical indexes. The lung adenocarcinoma samples were divided into three groups according to TNM stage: early stage (stage Ⅰ, 16 cases) and late stage (stage Ⅲ) ~ Ⅳ period, a total of 15 cases) two groups. The raw data was quality tested by dChip, normalized, and then analyzed for differential genes. 344 biological signal genes were obtained from the MsigDB database and signal enrichment analysis was performed by GSEA. [Result] The 14 differentially expressed genes, such as SEMA3, PLAU and CDKN2A, were obtained, and the differentially expressed genes were closely related to apoptosis and cell adhesion. We selected 344 genes from MsgDB database from Bicarta, KEGG and GenMAPP for enrichment analysis, and found that 22 pathways such as Death pathway, Leukocyte transendothelial migration and Focaladhesion were significantly enriched and enriched in advanced lung adenocarcinoma Pathways are mainly involved in apoptosis, cell adhesion and migration processes. [Conclusion] There are some obvious differentially expressed genes in early stage and late stage lung cancer, and some signal pathways are obviously enriched in advanced lung adenocarcinoma.