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目的探讨甲异靛对NB4细胞系的抑制增生、诱导凋亡和分化的作用。方法用锥虫蓝染色方法进行活细胞计数;用NBT还原实验和CD11a、CD11b的表达判定促分化作用;光镜和电镜细胞形态学、TUNEL标记和Sub-G1测定观察细胞凋亡,RT-PCR和流式细胞术分析法检测凋亡调控基因mRNA及蛋白水平表达。结果20μmol/L和30μmol/L甲异靛可明显抑制NB4细胞的增生,诱导细胞凋亡并下调bcl-2表达,使细胞停滞在G0/G1期。5μmol/L和10μmol/L时NB4细胞的NBT还原能力明显升高,CD11a表达明显升高。结论甲异靛通过诱导细胞凋亡和使细胞停滞在G0/G1期这两种机制显著抑制NB4细胞增生,此外,还有部分诱导细胞分化的作用。
Objective To investigate the inhibitory effect of meisoindigo on NB4 cell line and its role in inducing apoptosis and differentiation. Methods Cell viability was measured by trypan blue staining. NBT reduction assay and expression of CD11a and CD11b were used to determine the differentiation of cells. Apoptosis was observed by light and electron microscopy, TUNEL labeling and Sub-G1 assay. RT-PCR And flow cytometry analysis of apoptosis regulatory gene mRNA and protein expression. Results 20μmol / L and 30μmol / L mechloreol could significantly inhibit the proliferation of NB4 cells, induce apoptosis and down-regulate the expression of bcl-2, and arrest the cells in G0 / G1 phase. NB4 cells at 5μmol / L and 10μmol / L had significantly higher NBT reduction ability and higher CD11a expression. Conclusions Methyindigo significantly inhibits the proliferation of NB4 cells by inducing apoptosis and arresting the cells at G0 / G1 phase. In addition, there are some effects of inducing cell differentiation.