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目的探讨越橘花色苷对宫颈癌Hela细胞的作用机制。方法以不同浓度的越橘花色苷作用于宫颈癌Hela细胞48h后,通过Gimsa染色观察越橘花色苷抑制Hela细胞的形态学改变,通过流式双染观察Hela细胞凋亡情况,通过RT-PCR观察Hela内P53的表达,并测定Hela细胞内的SOD、GSH、MDA值。结果 Gimsa染色可见越橘花色苷可使Hela细胞光泽度下降,细胞数减少,稀疏,细胞变小、核固缩。流式双染可见,随着橘花色苷浓度升高,凋亡率升高。与对照组比较,越橘花色苷浓度达到30mg/ml时,P53的表达量最高,差异有统计学意义(P<0.05)。与对照组比较,30mg/ml和40mg/ml的越橘花色苷组SOD和GSH含量升高,差异有统计学意义(P<0.05)。结论越橘花色苷可通过上调抑癌基因P53的表达,增强细胞凋亡,并提高Hela细胞的抗氧化能力,从而达到抑制宫颈癌Hela细胞的作用。
Objective To investigate the mechanism of anthocyanins on human cervical carcinoma Hela cells. Methods Different concentrations of anthocyanins on human cervical cancer Hela cells 48h, Gimsa staining observed bilberry anthocyanins Hela cells morphological changes were observed by flow cytometry Hela cell apoptosis, by RT-PCR The expression of P53 in Hela cells was observed and the contents of SOD, GSH and MDA in Hela cells were measured. Results Gimsa staining showed that the bilberry anthocyanin Hela cells can reduce the gloss, reduced number of cells, sparse, smaller cells, nuclear pyknosis. Flow double staining shows that, with the increase of anthocyanin concentration, apoptosis rate increased. Compared with the control group, the expression of P53 was highest when the concentration of anthocyanin of bilberry reached 30mg / ml, the difference was statistically significant (P <0.05). Compared with the control group, the contents of SOD and GSH in the groups of 30 mg / ml and 40 mg / ml anthocyanins increased, the difference was statistically significant (P <0.05). Conclusion Bilberry anthocyanins can up-regulate the expression of tumor suppressor gene P53, enhance apoptosis and increase the anti-oxidative capacity of Hela cells, so as to inhibit the effect of cervical cancer Hela cells.