论文部分内容阅读
对来自新疆裕民、托里、青河等3个县的63株野生白灵菇(Pleurotus eryngiivar.tuoliensis)的遗传多样性进行了SCoT分析。9条SCoT引物共产生147条带,多态性条带比率为92.52%,平均多态性条带数为15.11。总样本的Nei’s基因多样性指数为0.261,Nei’s遗传一致度0.612～0.932,平均为0.772,这表明中国野生白灵菇具有丰富的遗传多样性。聚类分析和主坐标分析结果高度相似,几乎将供试样本完全按地理分布划分为3大类群,表明野生白灵菇的遗传多样性与地理分布密切相关。遗传距离分析表明,3个居群中,裕民居群与托里居群的亲缘关系最近,遗传距离为0.086;托里居群与青河居群的亲缘关系最远,遗传距离为0.156。相关性分析表明,遗传距离与直线地理距离和经向地理距离均不存在相关性,而与纬向地理距离存在明显的正相关关系。
The genetic diversity of 63 wild Pleurotus eryngiivar.tuoliensis from three counties of Yumin, Tori and Qinghe in Xinjiang was analyzed by SCoT. A total of 147 bands were generated with 9 SCoT primers. The percentage of polymorphic bands was 92.52%. The average number of bands was 15.11. The Nei’s gene diversity index of the total samples was 0.261, Nei’s genetic identity was 0.612 ~ 0.932, with an average of 0.772, which indicated that the Chinese wild Pleurotus eryngii had rich genetic diversity. The results of cluster analysis and principal coordinate analysis are highly similar, and the test samples are almost divided into three groups according to their geographical distribution, indicating that the genetic diversity of wild Pleurotus nebrodensis is closely related to the geographical distribution. Genetic distance analysis showed that the three populations had the closest genetic relationship with Tuoli population and the genetic distance was 0.086. The genetic relationship between Tuoli population and Qinghe population was the farthest, and the genetic distance was 0.156. Correlation analysis showed that there was no correlation between genetic distance and linear geographic distance and meridional geographic distance, but significant positive correlation with latitudinal geographic distance.