通过将反义ｂｃｌ２ 基因转染于Ｕ９３７ 细胞， 建立了Ｂｃｌ２ 蛋白表达受抑的Ｕ９３７ 细胞模型， 证实了模型细胞的增殖和存活表型无明显变化． 放线菌素Ｄ结晶紫试验和ＥＬＩＳＡ检测表明模型细胞上清中ＴＮＦα的活性和含量无明显变化， 小鼠胸腺细胞增殖试验表明模型细胞上清中ＩＬ１ 活性升高， 提示ｂｃｌ２ 的表达对ＴＮＦα的表达无明显影响， 但可能在一定程度上抑制了ＩＬ１ 的表达或活性． 探讨ｂｃｌ２ 对细胞因子表达的调节作用， 为其作用机制的研究积累了有益资料 The antisense bcl  2 gene was transfected into U937 cells to establish a U937 cell model with suppressed expression of Bcl  2 protein, which confirmed that there was no significant change in the proliferation and survival phenotype of the model cells. Actinomycin D  crystal violet test and ELISA test showed that the model cell supernatant TNFα activity and content no significant change in mouse thymocyte proliferation test showed that the model cell supernatant IL 1 activity, suggesting that bcl  2 Expression of TNFα no significant effect, but may to a certain extent, inhibit the expression of IL 1 or activity. To investigate the regulatory effect of bcl-2 on the expression of cytokines and to accumulate useful data for its mechanism of action