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稻瘟病菌对潮霉素（ＨｙｇｒｏｍｙｃｉｎＢ，简写为ＨｍＢ）较为敏感，当不含无机盐的再生培养基中ＨｍＢ浓度达到１００μｇ／ｍｌ时，稻瘟病菌２５３９ｗ原生质体的再生即被完全抑制。我们利用带有ＨｍＢ抗性基因的质粒（ｐＡＮ７－１），通过ＰＥＧ②融合法对其原生质体进行转化，获得了ＨｍＢ抗性转化子。转化子以两类形式出现：一类为大而生长稳定的菌落，另一类为小而生长不稳定的菌落，两类菌落产生频率分别为每μｇＤＮＡ３－４个和１０－２０个。将大菌落转化子转接到含有ＨｍＢ的新培养基上，仍可正常生长，但小菌落转化子却不能，说明前者为真正的转化子而后者为流产转化子。ＤＮＡ杂交分析显示转化是由于质粒ＤＮＡ整合到了２５３９ｗ的染色体ＤＮＡ上，整合可在染色体ＤＮＡ的不同位点上发生。受试的７个转化子尽管各自的整合形式不同，但均在选择与非选择性培养中保持稳定。
Magnaporthe grisea was more sensitive to hygromycin B (HmB). When the concentration of HmB in the medium without inorganic salts reached 100μg / ml, the regeneration of 2539w protoplasts of Magnaporthe grisea was completely inhibited. We transformed the protoplasts by PEG2 fusion using the plasmid with the HmB resistance gene (pAN7-1), and obtained HmB resistant transformants. Transformants appeared in two forms: one for large and stable colonies, the other for small and unstable colonies, with two colonies producing frequencies of 3-4 and 10-20 per μg of DNA, respectively. The large colony transformants were transferred to the new medium containing HmB, can still grow normally, but small colony transformants can not, that the former is a true transformant and the latter abortion transformants. DNA hybridization analysis shows that the transformation is due to the integration of the plasmid DNA into the 2539w chromosomal DNA, which can occur at different sites on the chromosomal DNA. The seven transformants tested remained stable in both selective and non-selective cultures despite their different forms of integration.