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【目的】观察通脉注射液对脑缺血再灌注大鼠大脑皮质神经细胞N-甲基-D-天冬氨酸受体1(NMDAR1)mRNA表达的影响。【方法】选用SD大鼠随机分为假手术组,模型组,通脉注射液高、中、低剂量组(中药高、中、低剂量组,剂量分别为2.72、1.36、0.68 g.kg-1.d-1)。除假手术组外,其他组均采用改良4血管结扎法复制大鼠全脑缺血模型,取各组大鼠大脑皮质,采用原位杂交法检测各组大脑皮质神经细胞NMDAR1 mRNA阳性细胞数和染色光密度(D)值。【结果】与假手术组比较,模型组大鼠大脑皮质神经细胞NMDAR1 mRNA阳性细胞数和D值均显著增高(P<0.01或P<0.001);与模型组比较,中药高、中剂量组阳性细胞数显著减少(P<0.01或P<0.001),中药高剂量组的D值显著降低(P<0.01),而中药中、低剂量组的D值和中药低剂量组的阳性细胞数无显著变化(P>0.05)。【结论】通脉注射液治疗缺血性中风的作用与其能抑制缺血后神经细胞NMDAR1 mRNA的异常表达,减弱兴奋性神经毒性引发的神经细胞损伤有关。
【Objective】 To observe the effect of Tongmai injection on N-methyl-D-aspartate receptor 1 (NMDAR1) mRNA expression in cerebral cortex of cerebral ischemia-reperfusion rats. 【Methods】 SD rats were randomly divided into sham operation group, model group and Tongmai injection high, medium and low dose groups (high, medium and low dose group, the dose was 2.72,1.36,0.68 g.kg- 1.d-1). In addition to the sham operation group, the other groups were using the modified 4 vascular ligation method to copy the rat cerebral ischemia model, taking the cerebral cortex of each group, using in situ hybridization to detect the NMDAR1 mRNA positive cells in each group and Dyeing density (D) value. 【Results】 Compared with the sham operation group, the number of NMDAR1 mRNA positive cells and the D value of cerebral cortex neurons in model group were significantly increased (P <0.01 or P <0.001). Compared with the model group, (P <0.01 or P <0.001). The D value of high-dose Chinese herbal medicine group was significantly decreased (P <0.01), but there was no significant difference between D group and low-dose Chinese herbal medicine group Change (P> 0.05). 【Conclusion】 Tongmai injection can reduce the abnormal expression of NMDAR1 mRNA in ischemic neuronal cells and attenuate the neuronal damage induced by excitatory neurotoxicity.