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为减轻雷公藤的毒副作用,我科试制了复方雷公藤冲剂,并用以治疗30例皮肤病,今报告如下。LD_(50)测定和对照试验LD_(50)测定昆明种小白鼠50只(由南京医学院动物房提供),用雷公藤乙醇浸出液,何首乌水煎醇沉液,回收乙醇,合并母液过滤,将滤液浓缩至每毫升含原药材12g备用。根据预试验测得LD_o为60g/kg,LD_(100)为240g/kg,按等比级数,将上两剂量间区划分为5个剂量组,每组10只动物,灌肠给药,药量为0.2 ml(10g),试验前禁食4小时,灌肠后24、48、72小时观察小鼠死亡情况,结果测得LD_(50)为113.5g/kg。对照试验:单用雷公藤组5只小鼠,按
In order to alleviate the toxic side effects of Tripterygium wilfordii, our department made a compound of Tripterygium wilfordii and used it to treat 30 cases of skin diseases. This report is as follows. LD_ (50) determination and control experiment LD_ (50) Determination of 50 Kunming species of mice (provided by the Animal College of Nanjing Medical College), with Tripterygium alcohol extract, Polygonum multiflorum decoction, recovery of ethanol, combined with the mother liquor filtration, The filtrate was concentrated to 12 g/ml containing the original medicine. According to the preliminary test, the LD_o was 60g/kg, and the LD_(100) was 240g/kg. The upper two doses were divided into five dose groups according to equal proportions, 10 animals in each group, enema administration, medicine The amount was 0.2 ml (10 g), fasted for 4 hours before the test, and the death of mice was observed at 24, 48, and 72 hours after the enema. The result showed that the LD 50 was 113.5 g/kg. Controlled trial: 5 mice in the triptolide group alone, according to