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目的:建立测定大鼠血浆中氯化两面针碱的高效液相色谱法,并研究氯化两面针碱与大鼠血浆蛋白的结合率。方法:平衡透析法结合高效液相色谱法,对氯化两面针碱的大鼠血浆蛋白结合率进行测定。结果:氯化两面针碱与内标完全分离,血浆中其他成分无干扰,在0.0319～2.0400mg·L-1范围内线性关系良好,低、中、高3种不同质量浓度的方法回收率>95%,日内、日间精密度均符合方法学要求。低、中、高3种药物浓度的血浆蛋白结合率分别为68.20%,68.81%,67.37%。结论:高效液相色谱法用于氯化两面针碱的生物样品测定简便、快速、准确、可靠。氯化两面针碱在大鼠血浆中具有中等强度的蛋白结合率,蛋白结合率与透析液的药物浓度无关。
Objective: To establish a high performance liquid chromatography method for determination of nitidine chloride in rat plasma and to study the binding rate of nitidine chloride with rat plasma protein. Methods: The plasma protein binding rate of nitidine chloride in rats was determined by equilibration dialysis combined with high performance liquid chromatography. RESULTS: The nitidine chloride was completely separated from the internal standard and there were no interferences in other components in the plasma. The linearity was good in the range of 0.0319 to 2.0400 mg·L-1. The recoveries of three different concentrations of low, medium and high concentrations were > 95%, intraday and interday precision meet the methodological requirements. The plasma protein binding rates of the low, medium, and high drug concentrations were 68.20%, 68.81%, and 67.37%, respectively. Conclusion: The HPLC method for the determination of nitidine chloride in biological samples is simple, rapid, accurate and reliable. The nitidine chloride has a moderately strong protein binding rate in rat plasma, and the protein binding rate is independent of the drug concentration of the dialysate.